miR-146a Suppresses SUMO1 Expression and Induces Cardiac Dysfunction in Maladaptive Hypertrophy

<b>Table I. </b>Calcium transient and contractility parameters of cardiomyocytes infected with Ad_pre-mir-146a or Ad_decoy-146a.<b> </b> <b>Top table</b>, isolated mouse cardiomyocytes were infected with Ad_pre-mir-146a (MOI=50) for 24h. Data represent the mean ± s.e.m. in all panels (n=100-150 cells/4 hearts). <b>Bottom table</b>, isolated mouse cardiomyocytes were infected with Ad_decoy-146a (MOI=50) for 24h. Data represent the mean ± s.e.m. in all panels (n=100-150 cells/4 hearts). <b>Table II. </b>Echocardiographic parameters of mice injected with rAAV9_pre-mir-146a. Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. <b>Table III. </b>Hemodynamic parameters of mice injected with rAAV9_pre-mir-146a.<b> </b> Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. Max dPdt, maximum dP/dt; Min dPdt, minimum dP/dt. <b>Table IV. </b>Echocardiographic parameters of mice injected with rAAV9_decoy-146a.<b> </b> Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. <b>Table V. </b>Hemodynamic parameters of mice injected with rAAV9_decoy-146a.<b> </b> Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. <b>Table VI. </b>Echocardiographic parameters of SUMO1 TG mice injected with rAAV9_pre-mir-146a.<b> </b> Data represent the mean ± s.e.m. of cardiac functional parameters 8 weeks post-injection. NL, Negative littermate; SUMO1 TG, Cardiac-specific Cre/loxP-conditional <i>Sumo1</i>-transgenic mice<b>Table VII. </b>Cardiac cellular composition. Isolated cardiac cells were sorted with FACS and analyzed. Data represent the mean ± s.e.m. (n=5).