Figure 3 raw data: Effect of PTH1R and PKA agonists on high plating density cultures.

Fig3A. The amount of cAMP was measured by EIA in 98 wells plated with RC cells and exposed to 0 to 100 nM PTHrP (1-36) for 10 min.Fig3B. The number of bone nodules in 8 wells following treatment of RC cell cultures with 0 to 1 mM DBcAMP and 0 to 10 µM forskolin (Fsk).Fig3C. The number of bone nodules in RC cell cultures. RC cells were grown in the absence (control) or presence of 50 nM PTHrP (7-34).

图3A：通过酶联免疫吸附测定法（EIA）测量了在98个培养有RC细胞的孔板中，经0至100纳摩尔PTHrP（1-36）处理10分钟后的cAMP含量。图3B：在RC细胞培养中，经0至1毫摩尔DBcAMP和0至10微摩尔 forskolin（Fsk）处理后，8个孔中形成的骨结节数量。图3C：RC细胞培养中形成的骨结节数量。RC细胞在无（对照组）或存在50纳摩尔PTHrP（7-34）的条件下生长。