RNAseq analysis of RBPMS, RBFOX2 and QKI knockdown in rat PAC1 pulmonary artery smooth muscle cells (SMCs)

Investigation of the regulation of RNA binding proteins RBPMS, RBFOX and QKI via the global-scale analysis of alternative splicing changes in rat smooth muscle aorta. Bulk mRNA-Seq was carried out after panel knockdown of RBFOX2, RBPMS and QKI in PAC1 cells. Overall design: RBPMS, RBFOX2 and QKI were parallel knockdown in PAC1 cells. RBPMS knockdown was treated with RBPMS-specific siRNA; RBFOX2 knockdown was treated with RBFOX2-specific siRNA; QKI knockdown was treated with QKI-specific siRNA. The nagative control was treated with control siRNA.