Data for: Atoh1 is required for the formation of lateral line electroreceptors and hair cells, whereas Foxg1 represses an electrosensory fate

In electroreceptive jawed fishes and amphibians, individual lateral line placodes form lines of neuromasts on the head containing mechanosensory hair cells, flanked by fields of ampullary organs containing electroreceptors - modified hair cells that respond to weak electric fields. Extensively shared gene expression between neuromasts and ampullary organs suggests that conserved molecular mechanisms are involved in their development, but a few transcription factor genes are restricted either to the developing electrosensory or mechanosensory lateral line. Here, we used CRISPR/Cas9-mediated mutagenesis in G0-injected sterlet embryos (Acipenser ruthenus, a sturgeon) to test the function of three such genes. We found that the 'hair cell' transcription factor gene Atoh1 is required for both hair cell and electroreceptor differentiation in sterlet, and for Pou4f3 and Gfi1 expression in both neuromasts and ampullary organs. These data support the conservation of developmental mechanisms betwe..., , # Data for: Atoh1 is required for the formation of lateral line electroreceptors and hair cells, whereas Foxg1 represses an electrosensory fate

Dataset DOI: [10.5061/dryad.fqz612k3s](10.5061/dryad.fqz612k3s)

## Description of the data and file structure

In this dataset you will find images of sterlet sturgeon (*Acipenser ruthenus*) larvae underlying the data reported in **Supplementary Table 1** of the preprint Minařík M, Campbell AS, Franěk R, Vazačová M, Havelka M, Gela D, Pšenička M, Baker CVH (2024) *bioRxiv* 2023.04.15.537030 (DOI: 10.1101/2023.04.15.537030) and **Supplementary File 1**, **Supplementary File 2** and **Supplementary File 3** of the paper Minařík M, Campbell AS, Franěk R, Vazačová M, Havelka M, Gela D, Pšenička M, Baker CVH (2025) *eLife TBC*

The dataset contains four main folders (zip archives):

**Supplementary_File_1_Tyr** corresponds to the *Tyr* crispant data in Supplementary Table 1 of the preprint and Supplementary File 1 of the paper (Breakdown by sgRNA m...,