Gene expression analysis of Trem1-deficient Kupffer cells subjected to carbon tetracloride (CCl4) in liver fibrogenesis.

Analysis of Kupffer cells at gene expression level. The hypothesis tested in the present study was that the expression of TREM-1 on Kupffer cells isolated from wild type mice subjected to single dose of carbon tetracloride and sacrificed 12 hours after injection influences the expression of multiple genes implicating in several signaling pathways. Results provide important information of the response of Trem1-sufficient and Trem1-deficient Kupffer cells to carbon tetracloride, such as inflammatory responsive genes, up-or-down regulated inflammation, acute liver injury and liver fibrogenesis. Total RNA isolated from purified Kupffer cells of Trem1-deficient mice injected with single dose of carbon tetracloride and sacrifced 12 hours after and compared to purified Kupffer cells of wild type mice treated with single dose of oil (control) or carbon tetracloride and sacrificed 12 hours after.