Comparison of hydrogen administration methods in the treatment of radiation-induced heart disease in rats

Ionizing radiation is a known risk factor for the occurrence and development of cardiovascular diseases, particularly in patients receiving thoracic radiotherapy. This exposure leads to oxidative stress and inflammation, which can damage cardiac tissue and vascular endothelium. Molecular hydrogen (H2) has been recognized for its therapeutic potential, including antioxidant effects. In this study, male Wistar rats were irradiated with a dose of 10 Gy (X-rays) in the chest area. Two and nine days post-irradiation, significant increases in lactate dehydrogenase (LDH), catalase, glutathione peroxidase activity, malondialdehyde, superoxide, and tumor necrosis factor alpha levels were observed in the rat blood plasma or heart tissue. Administration of H2 either via drinking H2-rich water (min. 4 mg/L) or inhaling H2 in air (4%), effectively decreased oxidative stress, LDH, and inflammatory proteins to normal levels. H2 also normalized the nuclear factor erythroid 2-related factor 2/Kelch-like..., , # Comparison of hydrogen administration methods in the treatment of radiation-induced heart disease in rats Dataset DOI: [10.5061/dryad.f4qrfj78s](https://doi.org/10.5061/dryad.f4qrfj78s) ## Description of the data and file structure **README – Dataset description** **Dataset title** Comparison of hydrogen administration methods in the treatment of radiation-induced heart disease in rats Dataset DOI: 10.5061/dryad.f4qrfj78s **1. Study overview** This dataset contains individual-level experimental data from a controlled animal study investigating the effects of different hydrogen administration methods on radiation-induced heart injury in rats. All measurements are provided at the level of individual animals. The dataset allows independent reanalysis and full reconstruction of statistical results reported in the manuscript.  **2. Experimental design** Animals were randomly assigned to four experimental groups: * Control (non-irradiated) * IR (irradiated) * HRW (irradiated +..., ,电离辐射是公认的心血管疾病发生与进展的危险因素，尤其在接受胸部放射治疗的患者中风险更为显著。此类辐射暴露可诱发氧化应激与炎症反应，进而造成心肌组织与血管内皮的损伤。分子氢（H₂）因其包括抗氧化作用在内的多种治疗潜力而受到广泛认可。本研究对雄性Wistar大鼠的胸部实施10 Gy剂量的X射线照射。在照射后第2天与第9天，研究人员在大鼠血浆与心脏组织中均观测到乳酸脱氢酶（LDH）、过氧化氢酶、谷胱甘肽过氧化物酶活性，以及丙二醛、超氧化物、肿瘤坏死因子-α（tumor necrosis factor alpha）水平的显著升高。通过饮用最低浓度为4 mg/L的富氢水，或吸入体积分数为4%的氢气两种方式给予分子氢，均可有效将氧化应激指标、LDH及炎症蛋白水平恢复至正常范围。此外，分子氢还可使核因子红细胞2相关因子2/Kelch样（nuclear factor erythroid 2-related factor 2/Kelch-like）相关指标恢复正常。 # 大鼠氢气给药方式对比治疗放射性心脏病研究 Dataset DOI: 10.5061/dryad.f4qrfj78s ## 数据与文件结构说明 **README – 数据集描述** **数据集标题** 大鼠氢气给药方式对比治疗放射性心脏病研究（Comparison of hydrogen administration methods in the treatment of radiation-induced heart disease in rats） Dataset DOI: 10.5061/dryad.f4qrfj78s **1. 研究概述** 本数据集包含一项对照动物实验的单只动物水平实验数据，该实验旨在探究不同氢气给药方式对大鼠放射性心脏损伤的干预效果。所有测量数据均以个体大鼠为单位提供。本数据集支持对已发表论文中报告的统计结果开展独立重分析与完整复刻。 **2. 实验设计** 实验动物被随机分配至4个实验组： * 对照组（未接受照射） * 照射组（IR） * 富氢水给药组（HRW，照射+……）