Genome-wide transcriptome analysis of human papillomavirus type 16 infected primary keratinocytes [monolayer]

HPV alters the host transcriptional program to establish infection and complete its lifecycle. Infection of keratinocytes has been difficult until our development of extracellular matrix (ECM) to cell transfer system that allows efficient infection of primary keratinocytes. Using this model, the transcriptional program of HPV infected keratinocytes at different times post infection was analyzed by mRNA-seq. mRNA profiles of control, HPV16-infected or HPV16- immortalized human foreskin primary keratinocytes were generated by deep sequencing, in triplicate, using Illumina.