Elemental ratios in Western Pacific sclerosponges

<p>A complete description of the collection and experimental methods is available in Grottoli et al. (2010). In brief, six <em>A. wellsi</em> sclerosponges were identified between 11.5 and 18 m depths along the wall at Short Drop Off, Palau. The specimens were stained with Alizarin Red on 26 July 2001, re-cemented onto the reef at a common depth of 11 m depth using Splash Zone marine epoxy, and allowed to grow out past the stain line for 2 years. In Saipan, three <em>A. wellsi</em> identified at 6 m depth and four <em>A. willeyana</em> sclerosponges identified between 7 and 9 m depth were stained with Alizarin Red on 15 July 2001, re-cemented onto the reef at a common depth of 8.3 m depth with marine epoxy, and allowed to grow out past the stain line for 2 years. On 15 July 2003 and 11 July 2003 all of the specimens were collected from Palau and Saipan, respectively, and were returned to the lab for further analysis.</p>本数据集的完整采集流程与实验方法已在Grottoli等人（2010）的研究中公开。简言之，在帕劳（Palau）的短落差潜点（Short Drop Off）崖壁上11.5至18米水深区间内，共鉴定得到6株韦氏星根硬海绵（*A. wellsi*，sclerosponge）；所有标本于2001年7月26日使用茜素红（Alizarin Red）染色，随后采用Splash Zone品牌海洋环氧胶（marine epoxy）将其重新固定于11米的统一水深礁体上，并使其生长至超出染色线后持续培养2年。在塞班岛（Saipan），于6米水深处鉴定得到的3株韦氏星根硬海绵（*A. wellsi*），以及7至9米水深区间内鉴定得到的4株威利星根硬海绵（*A. willeyana*，sclerosponge），均于2001年7月15日使用茜素红（Alizarin Red）染色，随后使用海洋环氧胶将其统一固定于8.3米水深的礁体上，并使其生长至超出染色线后持续培养2年。所有标本分别于2003年7月15日（帕劳）与2003年7月11日（塞班岛）被采集回收，并带回实验室开展后续分析。