hAEC cultured <i>in vitro</i> express genes associated with pluripotency.
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(A) Representative images of the electrophoresis of RT-PCR products of mRNAs for transcription factors OCT4 (151 bp), SOX2 (264 bp), NANOG (286 bp), REX1 (306 bp), KLF4 (134 bp) of hAEC cultured in vitro. L = ladder, H9 = hESC line H9 (positive control), P = passage. As negative control (-RT), the reverse transcriptase enzyme was not added. (B) Relative expression levels obtained from RT-qPCR of the pluripotency genes (mean from 5 independent experiments).
创建时间:
2016-03-03



