Soil Nitrogen, Water Content, Microbial Biomass, and Archaeal, Bacterial and Fungal Communities from the East River Watershed, Colorado collected in 2016-2017

This data set contains soil measurements made at the Pumphouse Hillslope to Floodplain transect at the locations of the early snowmelt-timing manipulation experiments in the East River Watershed in Colorado, USA. The data were collected in 2016 and 2017 to determine soil microbial responses to snow accumulation, snowmelt, and transition from winter to spring. Some additional data from the Washington Gulch snowmelt manipulation experiment lcoations are also included here. Data were collected on soil cores collected in the field at three soil depths (0 to 5 cm, 5 to 15 cm, 15 cm +) below the soil surface and analyzed in the on homogenized samples with roots and gravel removed. Data include gravimetric soil water content (48 hr oven-dried) and soil pH measurements (1:1 w/vol in ddH2O), dissolved nitrogen pools measured in soil extracts (2M KCl extractant), microbial biomass carbon and nitrogen (0.5M K2SO4 extractant), and archaeal, bacterial, and fungal species determined by Illumina 300 bp paired-read marker gene sequencing (16S rDNA for archaea and bacteria and ITS for fungi).本数据集包含美国科罗拉多州东河流域泵房山坡至漫滩样带的提前融雪时间调控实验点位的土壤测定数据。该数据采集于2016年与2017年，旨在探究土壤微生物对积雪累积、融雪过程以及冬春季节转换的响应特征。本数据集同时纳入了华盛顿谷融雪调控实验点位的部分补充数据。数据采集自野外获取的土壤芯样，测定时选取土壤表层以下三个深度层级的样品：0~5 cm、5~15 cm及15 cm以上，所有样品经均质化处理并去除根系与砾石后开展分析。数据集涵盖的测定指标包括：经48小时烘箱烘干法测定的重量法土壤含水量、以去离子水按质量体积比1:1（w/vol）制备浸提液后测定的土壤pH值、以2M氯化钾浸提剂得到的土壤浸提液中溶解态氮库含量、以0.5M硫酸钾浸提剂得到的微生物生物量碳与氮含量，以及通过Illumina 300 bp双端标记基因测序（Illumina 300 bp paired-read marker gene sequencing）鉴定得到的古菌、细菌与真菌物种组成，其中古菌与细菌采用16S核糖体DNA（16S rDNA）作为标记基因，真菌采用内转录间隔区（ITS，Internal Transcribed Spacer）作为标记基因。