Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments.

<p>Mussel larvae of Mytilus galloprovincialis were grown in two pH treatments (pH 8.1 and 7.4). Larvae were collected for biological measurements of shell field development and calcification at 35 hours post-fertilization (hpf, trochophore stage). Calcein dye was added to the cultures prior to the start of calcification. Calcofluor is live dye and so was added to sampled larvae at 35 hpf for immediate imaging. Confocal microscopy was used for 3D imaging of larvae. Images were processed in ImageJ. Shell field area was determined as the area stained by calcofluor, on one valve. Calcification area was determined as the area stained by calcein, on one valve. See publication for details.&nbsp;</p>地中海贻贝（Mytilus galloprovincialis）的幼体在两种pH处理组（pH 8.1与pH 7.4）中培育。于受精后35小时（hpf，即担轮幼虫期）收集幼体，用于开展壳场发育与钙化过程的生物学检测。钙黄绿素（Calcein）染色剂于钙化启动前被添加至培养体系。荧光增白剂（Calcofluor）为活体染色剂，故在35 hpf时被添加至取样幼体以完成即时成像。采用共聚焦显微镜（Confocal microscopy）对幼体开展三维成像。图像经ImageJ软件处理完成。壳场面积以单个壳瓣上被荧光增白剂染色的区域进行定量。钙化面积以单个壳瓣上被钙黄绿素染色的区域进行定量。详细实验细节参见已发表文献。