qRTPCRs in different cell lines on a panel of genes exhibiting altered transcript levels in the microarray expression screen in U-251 MG cells after NFIX-siRNA treatment.

Except for HSF1, only statistically significant (T-test, p value<0.05) changes in expressions are shown. Non-significant changes are shown as dashes. For HSF1, all changes are significant except U-251 MG and U-343 MG-Cl2:6. All values are average ±S.D. of the ratios of values for NFIX-siRNA over control-siRNA, calculated by delta-delta Ct method using GAPDH as internal control. For some reactions, ATCB was also used as internal control and similar values were obtained (not shown).