Transcriptome profiling of gingival fibroblasts treated by vitamin D3

In humans, vitamin D3 is a secosteroid, a prohormonal precursor of biologically active hydroxyforms. Vitamin D3 is related to periodontitis. The third American Health and Nutrition Survey found that plasma 25(OH)D3 levels are negatively correlated with attachment loss in people aged >50 years. The relationship between vitamin D3 and periodontitis remains to be investigated. We sent human gingival fibroblasts treated by vitamin D3 for transcriptome sequencing. Comparative gene expression profiling analysis of RNA-seq data for human gingival fibroblasts.The cells from one participant were split into two groups; one group received vitamin D3 as stimulation for 48 hours, while the other group received 0.1% ethanol as a control. Each group included three biological repetitions.