Label-free proteomic analysis of hepatocyte spheroids fed physiologically relevant levels of palmitic acid and oleic acid to mimic non-alcoholic fatty liver disease.

We have grown hepatocyte spheroids for 21 days following an established protocol. We have carried out proteomic analysis of the difference between control and treated (Fatty acids) spheroids during seven days (5 timepoints at day 0, 1, 2, 4 and 7). Samples were digesting using filter aided sample preparation-based digestion and subsequently analysed using label free LC-MS/MS.