Induction of a colitogenic phenotype in Th1-like cells depends on interleukin-23 receptor signaling [cytokine cultures]

RNA-sequencing of in vitro differentiated T cells cultured with varying cytokine conditions: IL-12, IL-12+IL-21, IL-1b+IL-6+IL-23, and TGFb+IL-6 Naive T cells were isolated from reporter mice for IL-23R that carry an IRES-eGFP in the Il23r locus. Naive T cells (CD44lowCD62LhiCD4+CD25-) were isolated from heterozygous mice (Il23r wt/eGFP) by fluoresence-activated cell sorting (FACS) and then cultured for 5 days with full medium containing the aforementioned cytokine conditions. Cells were then sorted based on eGFP expression before rna-sequencing. The reporter allele (Il23reGFP) represents a functional KO of IL23r combined with eGFP through an IRES element. Therefore, GFP was used as a indicator for IL23r expression in cells.