Quantitative Analysis of Transcriptomes of small molecules generated Neural Precursor Cesll (smNPCs) and smNPCs-derived neurons by next generation sequencing

The aim of this next generation sequencing is to analyze the difference between smNPC and smNPC derived neurons by RNA-sequencing. In this study, the smNPCs were generated from inducible pluripotent stem cells (iPSCs) derived from human cord blood cells. The obtained smNPCs were further differentiated into neurons using a protocol that stems from Stuart M Chambers et al (2012, Nature Biotech), which described a system to differentiate human embryonic stem cells into nociceptive sensory neurons and Peter Reinhardt et al (2013, Plos one), which developed a protocol to generate human peripheral neurons from smNPC. Briefly, a peripheral sensory lineage was induced from smNPCs by activating WNT signaling, while inhibiting NOTCH and FGF prior to BMP4 treatment. Neuronal differentiation was further enhanced by treatment with dbcAMP and other growth factors until 60 days post differentiation (dpd). The neurons at 60 dpd are termed hiNeurons. In this study, the transcriptomes of hiNeurons and smNPCs were quantitatively analyzed by RNA-seq. Investigate the differential gene expression before (smNPCs) and after (hiNeurons) differentiation