Supplementary file 4, Expression level shown as FPKM (Fragments Per Kilobase of transcript/gene per Million mapped reads)

This study focuses on understanding how plants, specifically Arabidopsis thaliana, respond to two major pollutants, lead (Pb) and vanadium (V) found in oil contaminated land. While the effects of these pollutants on animals are well-studied, their impact on plants is not as clear. To fill this gap, we exposed Arabidopsis seedlings to Pb and V and analyzed the changes in gene expression using advanced sequencing and validated the data using RT PCR technique.
The results showed that exposure to Pb and V led to significant changes in gene expression in Arabidopsis seedlings compared to untreated samples. Through Gene Ontology and pathway analysis, we identified several biological processes and pathways associated with the differentially expressed genes. Overall, this investigation sheds light on the molecular mechanisms underlying the toxicity and tolerance of Arabidopsis to Pb and V, providing valuable insights into how plants respond to heavy metal stress.

本研究旨在解析植物——尤以拟南芥（Arabidopsis thaliana）为对象——对石油污染土壤中两种主要污染物铅（Pb）与钒（V）的响应机制。尽管此类污染物对动物的影响已得到广泛研究，但其对植物的作用效应仍未明确。为填补这一研究空白，我们将拟南芥幼苗暴露于铅与钒胁迫环境中，采用高通量测序技术分析其基因表达变化，并通过逆转录聚合酶链式反应（RT PCR）对数据进行验证。结果表明，与未处理对照组相比，铅与钒暴露可使拟南芥幼苗的基因表达发生显著改变。通过基因本体论（Gene Ontology）与通路富集分析，我们鉴定出多个与差异表达基因相关的生物学过程及调控通路。综上，本研究阐明了拟南芥应对铅与钒胁迫的毒性耐受分子机制，为解析植物响应重金属胁迫的分子通路提供了宝贵的科学见解。