The origin, deployment, and evolution of a plant-parasitic nematode effectorome

Plant-parasitic nematodes constrain global food security. During parasitism, they secrete effectors into the host plant from two types of pharyngeal gland cells. These effectors elicit profound changes in host biology to suppress immunity and establish a unique feeding organ from which the nematode draws nutrition. Despite the importance of effectors in nematode parasitism, there has been no comprehensive identification and characterisation of the effector repertoire of any plant-parasitic nematode.
To address this, we advance techniques for gland cell isolation and transcriptional analysis to define a stringent annotation of putative effectors for the cyst nematode Heterodera schachtii at three key life-stages. We define 659 effector gene loci: 293 “known” high-confidence homologs of plant-parasitic nematode effectors, and 366 “novel” effectors with high gland cell expression. In doing so we define a comprehensive “effectorome” of a plant-parasitic nematode.
Using this effector definit..., Instructions for the generation of transcriptional networks are available here: https://github.com/BethMolloy/Effectorome_H_schachtii, , # The origin, deployment, and evolution of a plant-parasitic nematode effectorome.

[Access this dataset on Dryad] (DOI:10.5061/dryad.rfj6q57hn)

Data associated with the effectorome of the plant-parasitic nematode Heterodera schachtii. Principally annotated transcriptional network files, and computationally predicted structures of all Heterodera schachtii and Globodera rostochiensis proteins (using Alpha fold and ESM fold).

## Description of the data and file structure

1. H. schachtii transcription factors predicted as described in Molloy et al. (H.schachtii_tfs.names.withzfc4.376.names)

2. Transcriptional network files showing correlations in gene expression across nematode infection (as outlined in Siddique et al. 2022) - above a threshold correlation co-efficient of 0.975 - between genes encoding:

i. H. schachtii effectors (659_effectors_network_BM_0.975.gephi; 659_effectors_network_BM_0.975.gexf)
ii. H. schachtii transcription factors and effectors (3d_network_TFs_and...