A proteome-wide screen identifies the calcium binding proteins, S100A8/S100A9, as clinically relevant therapeutic targets in aortic dissection: a translational study [scRNA-seq]

S100A8 and S100A9 (aliases MRP8 and MRP14) are highly expressed in neutrophils and monocytes and are classified as damage-associated molecular pattern (DAMP) molecules or alarm molecules. However, the role of S100A8/A9 in aortic dissection has not been reported. In the present study, by employing an unbiased proteomics approach and RNA sequencing analysis , we found that the protein expression of calcium binding proteins S100A8/A9 were upregulated in the aorta and serum of TAAAD patients and also in a mouse model. Overall design: We performed a single-cell RNA sequencing analysis of aorta samples from mice AD model. AD model was established as follows: three-week-old male mice were conventionally fed normal chow and given BAPN (A3134, Sigma-Aldrich, St. Louis, Missouri, USA) in drinking water (1 g/kg/d)) for 4 consecutive weeks, and Paquinimod (ABR 25757, T7310, Targetmol, Shanghai, China) (10 mg/kg/d) was given via intragastric administration until experimental termination.