Yuting Sun, Yejing Ge, Jenny Drnevich, Yong Zhao, Mark Band, Jie Chen (2011) CIL:13593, Mus musculus, myoblast. CIL. Dataset

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiate in the presence of 50nM rapamycin (Rap) for 3 days. 25nM TSA was added when the cells were ~60% confluent and removed the next day upon induction of differentiation. TSA was able to rescue the effect of Rap to allow myotube maturation.

This image is the control of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13592, 13594, 13601.

本研究选取了C2C12细胞系，一种小鼠肌原细胞系，用以探讨肌生成分化过程中的调控因子。在分化培养基中培养3天后，这些细胞分化为含有多个核（蓝色）的肌管（绿色）。稳定表达 FLAG 标记的拉帕霉素抵抗性、激酶失活性（RR/KI）mTOR的C2C12细胞，在50nM拉帕霉素（Rap）存在下诱导分化3天。当细胞约达60%融合时，加入25nM TSA，次日分化诱导后去除。TSA能够挽救拉帕霉素的效果，从而促进肌管的成熟。此图像为JCB 189: 1157-1169, 2010中第9B图不同处理方法的对照组。亦请参阅CIL: 13592, 13594, 13601。