Effect of the number of I-SceI sites on the counter-selection for large-scale deletiona.

aData are means ± SD from three independent experiments for genetic manipulations.b20.5 kb-DEL, large deletion of 20.5 kb DNA sequence (528148–548697 SubtiList coordinates) of BUK; 75.9 kb-DEL, large deletion of 75.9 kb DNA sequence (1781306–1857233 SubtiList coordinates) of BUK.cThe first double-crossover recombination efficiency was calculated as the number of CmR colonies/µg of dsDNA PCR product.dCounter-selection efficiency was calculated as Nr/Nt. Nr, number of 5FUR CmS colonies in the culture treated by different concentration of xylose; Nt, number of total colonies in culture treated by different concentration of xylose.