On- and off-target mutations in viral genomes induced by Cas9:gRNA-206 in the HSV1 progeny virus.
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OTC indicates an off-target candidate (with the number of sites as shown in Supplementary Tables S5, S6). Mismatches in the target sequence (20-nt gRNA206 hybrid region and 3-nt PAM sequence) are bolded and underlined. Mean indel mutation frequencies were determined using deep sequencing (N = 3). N.D., none detected. The sequence locus indicates the position on HSV1 genome (GenBank: NC_001806.1). (−), negative strand; (+), positive strand.
创建时间：
2015-12-02
