GAPDH assembly (ace) file

De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484针对靶向甘油醛-3-磷酸脱氢酶（glyceraldehyde-3-phosphate dehydrogenase, GAPDH）的2个混合细菌人工染色体（bacterial artificial chromosome, BAC）克隆的焦磷酸测序（pyrosequencing）数据开展从头组装（de novo assembly），所用测序平台为454 Genome Sequencer FLX系统，采用长读长GS FLX Titanium测序化学试剂。实验使用罗氏（Roche，瑞士巴塞尔）开发的GS De novo Assembler软件进行组装，参数设置如下：启用大型/复杂基因组选项，以pCC1BAC HindIII载体序列作为修剪数据库，以大肠杆菌（Escherichia coli）菌株K12亚种DH10B的基因组序列（GenBank登录号CP000948）作为筛选数据库，最小重叠长度设为200 bp，最小重叠相似度设为98%，最小重叠群（contig）长度设为500 bp。原始数据可在美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的序列读取档案（Sequence Read Archive）中获取，编号为SRP003484。