Fig 2 Qrfp-/- mice are lean and hypophagic.

A, B, Body weights of male (A) and female (B) Qrfp-/- mice (red lines) under ad lib feeding of normal chow compared with wild type littermates (blue lines) plotted over time. C, D, Body weights of male (C) and female (D) Qrfp-/- mice (red lines) under feeding a high-fat diet compared with wild type littermates (blue lines). High-fat diet contained 56.7% fat (High Fat Diet 32, CLEA Japan Inc., Tokyo, Japan). E, Left panel, Representative CT images of age-matched (15 weeks) wild type (upper panel) and Qrfp-/- mice. Fat mass is shown in yellow. Right panel, Lean and fat mass of Qrfp-/- mice compared with wild type littermates (n=5). *, pnormalized by 0.75 power of body weight. Right panel, Cumulative food intake for 24 weeks (9 to 33 weeks of age) of Qrfp-/- mice (closed bars) under ad lib feeding of normal chow compared with wild type littermates (open bars). G, Basal energy expenditure of Qrfp-/- mice (closed bars, n=6) is comparable to that of wild type mice (open bars, n=6). Left panel, VO2 normalized by 0.75 power of body weight. Middle panel, VCO2 normalized by 0.75 power of body weight. Right panel, Respiratory quotient (RQ) of wild type mice vs Qrfp-/- mice. H, Daily locomotor activity of Qrfp-/- mice, which was comparable to that of wild type mice.