Additional file 2 of Fine dissection of limber pine resistance to Cronartium ribicola using targeted sequencing of the NLR family

Additional file 2: Table S1. PCR primers and gene sequences for amplicon-based targeted sequencing of limber pine resistance gene analogs (RGAs). Table S2. Summary of targeted-seq of limber pine RGAs and their SNPs detected for genetic map construction in the seed family LJ-112. Table S3. RGAs and their SNPs mapped by amplicon-based targeted genomic sequencing in seed family LJ-112. Table S4. Summary of functional genes and their representative SNPs genetically mapped in seed family LJ-112 in the present and previous studies. Table S5. Oligonucleotides designed for TaqMan-based SNP genotyping. Table S6. Syntenic analysis of Cr4-linked NLRs with sugar pine genome sequences. Table S7. DNA and protein sequences of functional genes so far genetically mapped in limber pine seed families.

附加文件2：表S1。用于柔枝松（limber pine）抗病基因同源序列（resistance gene analogs, RGAs）基于扩增子的靶向测序的聚合酶链式反应（Polymerase Chain Reaction, PCR）引物及基因序列。表S2：种子家系LJ-112中柔枝松RGAs靶向测序概况，及其用于遗传图谱构建的检测单核苷酸多态性（single nucleotide polymorphism, SNP）汇总。表S3：在种子家系LJ-112中通过基于扩增子的靶向基因组测序定位得到的RGAs及其SNP位点。表S4：本研究及既往研究中，在种子家系LJ-112内完成遗传定位的功能基因及其代表性SNP位点汇总。表S5：用于基于TaqMan的SNP基因分型的寡核苷酸。表S6：与Cr4连锁的核苷酸结合富亮氨酸重复受体（Nucleotide-binding leucine-rich repeat receptors, NLRs）与糖松基因组序列的共线性分析。表S7：目前已在柔枝松种子家系中完成遗传定位的功能基因的DNA与蛋白质序列。