Transcriptome analysis of avocado embryogenic cultures selected for resistance to Rosellinia necatrix exudates

Embryogenic cultures derived from a zygotic embryo of the avocado cv. Anaheim, were selected for resistance to the culture filtrate (CF) of Rosellinia necatrix, the causal agent of avocado white root rot. Cultures were obtained through recurrent selections in progressively increasing concentrations of fungal CF (from 20% up to 80%). RNA-Seq technology was used to compare the transcriptomic profiles of the avocado callus tolerant line L3 (capable to survive in the presence of 80% CF) and control line AN-9 (not exposed to CF), after 24 hours of growth in a medium containing 40% CF.