Data for: Evidence to support phytosanitary policies - The minimum effective heat treatment parameters for pathogens associated with forest products

Research on reducing the movement of pests on wood products has led to several options for safer trade including heat treatment of wood to mitigate pests. In this study, pathogenic organisms commonly regulated in the trade of forest products were tested to determine the minimum heat dose (temperature and time) required to cause mortality. The mycelial stage of tree pathogens, Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis, which may be found in wood products, were tested in vitro using the Humble water bath with parameters simulating the rate of heat applied to wood in a commercial kiln. The lethal temperature for the pathogens ranged from 44 to 50°C for a 30-minute treatment duration. A molecular diagnostic method to confirm pathogen mortality was available for five of the species heat treated. RNA detection using reverse transcription real-time PCR was used to validate pathogen mortality fol..., The data are observations on post-treatment survival of Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis exposed to heat in a water bath. The experiments were used to determine the optimal heating conditions for treatment of wood infested with forest pathogens. The mycelial stage of pathogens growing on grain were exposed to temperatures from 40 up to 52 degrees celsius for 30 minutes. Heat-treated and unexposed control grains with mycelium were plated on 20% V8 agar for Phytophthora species or 2% malt extract agar with yeast extract for fungi and observed for growth to determine if they survived treatment at each of the exposure temperatures., , # Data for: Evidence to support phytosanitary policies -The minimum effective heat treatment parameters for pathogens associated with forest products [https://doi.org/10.5061/dryad.7m0cfxq3k](https://doi.org/10.5061/dryad.7m0cfxq3k) Data are observations on post-treatment survival of *Heterobasidion occidentale*, *Grosmannia clavigera*, *Bretziella fagacearum*, *Phytophthora cinnamomi*, *P. lateralis*, *P. ramorum* and *P. xmultiformis* exposed to heat in a water bath at temperatures ranging from 40 up to 52 degrees Celsius for 30 minutes. # Description of the data and file structure These data were assembled and summarized from raw data sheets and transferred into excel format. Description of fields: * Experiment number: The experimental run within which the data were collected * Experiment date: The date on which the experimental run was conducted * Species: The Latin name of the pathogen containing the first initial of the genus followed by the species name * Isolate...针对降低木制品上有害生物移动的研究催生了多种更安全的贸易方案，其中包括通过热处理木材以减轻有害生物危害。本研究针对林产品贸易中通常受监管的病原微生物开展试验，以确定导致其死亡所需的最低热处理剂量（温度与时间）。本试验针对木制品中可能存在的树木病原菌菌丝体阶段，即西方异担子菌（Heterobasidion occidentale）、棒形喙孢霉（Grosmannia clavigera）、板栗枯萎病菌（Bretziella fagacearum）、樟疫霉（Phytophthora cinnamomi）、侧生疫霉（P. lateralis）、栎树猝死病菌（P. ramorum）以及多形疫霉（P. xmultiformis），采用恒温水浴箱（Humble water bath）进行体外试验，试验参数模拟商用窑炉对木材的加热速率。经30分钟处理后，这些病原菌的致死温度区间为44至50℃。针对其中5个热处理后的菌种，可采用分子诊断方法确认其是否死亡。通过反转录实时聚合酶链式反应（reverse transcription real-time PCR）进行RNA检测以验证病原菌是否死亡…… 本数据集记录了西方异担子菌、棒形喙孢霉、板栗枯萎病菌、樟疫霉、侧生疫霉、栎树猝死病菌及多形疫霉经恒温水浴加热处理后的存活情况观测数据。本试验旨在确定侵染森林病原菌的木材的最优热处理条件。将生长在培养基上的病原菌菌丝体分别置于40至52℃的环境中处理30分钟。将经热处理及未暴露的带菌丝体对照培养基分别接种至疫霉属菌种专用的20% V8琼脂培养基，或真菌专用的含酵母提取物的2%麦芽浸膏琼脂培养基上，观察其生长情况以判断在各暴露温度下病原菌是否存活。 # 数据集所属：支撑植物检疫政策的证据——林产品相关病原菌的最低有效热处理参数 [https://doi.org/10.5061/dryad.7m0cfxq3k](https://doi.org/10.5061/dryad.7m0cfxq3k) 本数据集记录了西方异担子菌、棒形喙孢霉、板栗枯萎病菌、樟疫霉、侧生疫霉、栎树猝死病菌及多形疫霉经恒温水浴加热处理后的存活情况观测数据，处理温度区间为40至52℃，处理时长为30分钟。 # 数据与文件结构说明 本数据集从原始数据记录表中整理汇总，并转换为Excel格式。 字段说明： * 试验编号：采集数据的试验批次 * 试验日期：试验批次开展的日期 * 菌种：病原菌的拉丁学名，格式为属名首字母缩写+菌种名 * 菌株……