Summary of SNP discovery and validation.

The number of primers that amplified a single product are shown for the first validation procedure, PCR test. The number of primer pairs that had melt curves with putative SNPs are shown for HRMA validation. The Sanger sequencing validation procedure shows the number of sequenced HRMA products that confirmed the SNPs identity for a primer pair. SNPs that were successfully genotyped from these sequences in a 5′-nuclease genotype are shown for the final validation procedure.1Seeb et al. 2011 [37].2Everett et al. 2011 [38].