Ambient RNA analysis of single-nuclei RNA-Seq data from the frontal cortex of C57BL/6J mice

Contamination of ambient RNA was found in single-nuclei RNA-Seq data of mouse frontal cortex. CellBender plus subcluster cleaning selectively removed ambient RNA signature from glial cell types. P56 wildtype mice were harvested rostral part of the frontal cortex which is corresponding to the cortex part of Coronal level 22-36 in Allen Brain Atlas: Reference Atlas, Version 2 (2011), and extracted single nuclei for sequencing.