Capillary differences with age and fiber type are attenuated by accounting for fiber shape

This dataset contains quantitative measurements of skeletal muscle fiber morphology and capillarization in young and older women. Muscle biopsies were obtained from both a rested leg and a leg that had performed a single bout of heavy resistance exercise five days prior to sampling. Variables include muscle fiber cross-sectional area, perimeter, and Shape Factor Index (SFI), as well as multiple indices of capillarization, including capillary density, capillary-to-fiber ratio, individual capillary-to-fiber ratio, and morphology-adjusted capillarization metrics. The dataset enables analysis of age-, fiber type-, and condition-related differences in muscle fiber geometry and capillary supply. , , # Capillary differences with age and fiber type are attenuated by accounting for fiber shape Dataset DOI: [10.5061/dryad.9p8cz8wwr](10.5061/dryad.9p8cz8wwr) ## Description of the data and file structure This dataset derives from a study of skeletal muscle capillarization in young and older women. Muscle biopsies were collected from both legs of each participant. One leg performed a single bout of heavy resistance exercise five days prior to sampling (Exercised leg), while the contralateral leg served as the rested control (Rested leg). Each sample is identified by a unique alphanumeric sample ID corresponding to participant and leg. Muscle biopsies were embedded, frozen, cryosectioned, and immunohistochemically stained for type I myosin heavy chain (fiber type identification), laminin (fiber borders), and CD31 (capillaries). From these sections, muscle fiber cross-sectional area, perimeter, and the derived Shape Factor Index (SFI) were quantified. Capillarization was evaluated using..., All participants provided explicit written consent for their data to be made publicly available in de-identified form. Prior to sharing, all direct identifiers were removed. The resulting dataset contains no information that could be used to re-identify individuals.本数据集包含年轻与老年女性骨骼肌纤维形态及毛细血管化的定量测量数据。所有样本均取自两类腿部：静息腿部，以及采样前5天完成单次大强度抗阻运动的腿部。 变量涵盖肌纤维横截面积、周长及形状因子指数（Shape Factor Index, SFI），同时包含多项毛细血管化相关指标，如毛细血管密度、毛细血管-肌纤维比值、单个毛细血管-肌纤维比值，以及经形态学校正的毛细血管化评估指标。本数据集可用于分析年龄、肌纤维类型及运动状态相关的肌纤维形态与毛细血管灌注差异。 # 纳入肌纤维形态因素后，年龄与肌纤维类型相关的毛细血管差异会被弱化 数据集DOI：[10.5061/dryad.9p8cz8wwr](10.5061/dryad.9p8cz8wwr) ## 数据与文件结构说明 本数据集源自一项针对年轻与老年女性骨骼肌毛细血管化的研究。所有受试者的双腿均采集了肌肉活检样本：其中一侧腿部在采样前5天完成单次大强度抗阻运动（运动腿部），对侧腿部作为静息对照组（静息腿部）。每个样本均配有唯一的字母数字组合样本ID，对应受试者及采集腿部。 肌肉活检样本经包埋、冷冻、冰冻切片后，针对I型肌球蛋白重链（用于肌纤维类型鉴定）、层粘连蛋白（用于标记肌纤维边界）及CD31抗原（用于标记毛细血管）进行免疫组化染色。基于上述切片，研究人员对肌纤维横截面积、周长及衍生的形状因子指数（Shape Factor Index, SFI）进行了定量分析，并采用[原文缺失]方法评估毛细血管化情况。所有受试者均签署了书面知情同意书，同意将其去标识化的数据公开共享。数据共享前，所有直接识别信息均已移除，最终数据集无任何可用于重新识别个体的信息。