Features of cloned and functionally tested monoclonal antibodies.

Monoclonal antibodies derived from iDCgB immunized mice (DC, n = 4), HCMV infected mice (CV, n = 2), and iDCgB immunized and protected against HCMV (n = 3). Control antibodies SM5-1 and KIOVIG are shown as references. CB unit = cord blood units used for humanization. EC50 binding capacity to recombinant gB protein (μg/ml) was calculated for clones DC06, DC14 and DC16, PR28 and PR32. IC50 HCMV-neutralizing capacity (μg/ml) was calculated for clones DC06, DC16 and PR32. V, D and J genes depict top matches according to IgBLAST and IMGT nomenclature. Amino acid (AA) mutations include substitutions, insertions, and deletions. Characterictics of heavy chains are depicted.