Antimicrobial cross-streak activity screening for seaweed-associated bacteria

Photographs taken of cross-streak agar plates with a seaweed-associated bacterial strain streaked in the middle of the plate, incubated for 7 days at 22 degrees Celsius (optimal growth temperature of seaweed-associated bacteria), and the yeast or bacterial test strains (pathogens) streaked from the outer edge of the agar plate up to the bacterial growth in the middle. Plates are incubated at 30 or 37 degrees Celsius (optimal growth temperature of the pathogenic strains) for 24hrs and inspected for activity. Gaps between the pathogen streak and the central growth streak serve as an indicator that the seaweed-associated bacterium produced a bioactive compound that inhibited the growth of the pathogen. Images are labelled as follow:bacterial strain name_media type_Candida_37C_agar OR bacterial strain name_media type_Candida_30C_agar OR bacterial strain name_media type_Bacteria_agarIn some instances, the media type is listed before the bacterial strain name.<br>The bacterial strain name will vary, while the media type could be M19 (g/L: 20 mannitol, 10 casamino acids, 20 peptone, 20 bacteriological agar, pH7.0) or ISP2 (g/L: 10 malt extract, 4 yeast extract, 4 glucose, 20 bacteriological agar, pH7.0), or ISP2+ or M19+ (media prepared in a commercial Red Sea salt solution at 38.2 g/L). 'Candida_37 or 30C' denotes that the analysis was performed on yeast test strains that have an optimal growth temperature of 37 or 30 degrees Celsius, 'Bacteria' denotes that the analysis was performed on bacterial pathogens, while 'agar' refers to the side of the plate visualized in the photograph.<br>The yeast test strains (37C) streaked on the plate are in the following order:On the left:<i>Candida auris</i> DSM 21092On the right, top to bottom:<i>Candida albicans</i> ATCC 24433, and <i>Candida albicans</i> ATCC 90028<br>The yeast test strains (30C) streaked on the plate are in the following order:On the left, top to bottom:<i>Candida </i><i>krusei</i><i> </i>ATCC 34135, and C<i>andida krusei</i> ATCC 14243On the right, top to bottom:<i>Candida tropicalis</i> ATCC 750, and <i>Candida tropicalis</i> ATCC 13803<br>The bacterial test strains (pathogens) streaked on the plate are in the following order:On the left, top to bottom:<i>Acinetobacter baumannii</i> ATCC 19606, <i>Klebsiella quasipneumoniae</i> ATCC 700603, <i>Staphylococcus aureus</i> ATCC 33591 (MRSA), <i>Enterococcus faecalis</i> ATCC 29212, and <i>Enterococcus faecalis</i> ATCC 51299 (VanB)On the right, top to bottom:<i>Acinetobacter baumannii</i> ATCC 1605, <i>Enterobacter cloacae</i> ATCC 1143, <i>Escherichia coli</i> ATCC 25922, <i>Pseudomonas aeruginosa </i>ATCC 27853, <i>Staphylococcus aureus </i>ATCC 29213