Fig_5a_100Ind_SwitchBack_Strategy_iii_2ndPhase_1

The microscopy images of the data in Fig. 5a for the following condition: 2 hours of CRISPRi induction (100% induction level) followed by inducers removal and the addition of 60 nM of AHL in the new medium. There are 61 microscopy stage positions with three channels each: phase-contrast bright field, mVenus, mRFP. The images were binned (2x2).

本数据集包含对应图5a中如下实验条件的显微图像：先开展2小时的CRISPR干扰（CRISPRi）诱导（诱导水平达100%），随后移除诱导剂，并在新培养基中加入60 nM的AHL。该数据集涵盖61个显微载物台成像位点，每个位点包含三个成像通道：相衬明场（phase-contrast bright field）、mVenus通道、mRFP通道。所有图像均经过2×2像素合并（binning）处理。