Establishment of a quantitative detection system for endotoxin
收藏科学数据银行2024-11-04 更新2026-04-23 收录
下载链接：
https://www.scidb.cn/detail?dataSetId=OA_1b746fec4b654222bddef9352c7bd800
下载链接
链接失效反馈官方服务：
资源简介：
Objective To prepare monoclonal antibody to bacterial endotoxin and establish a chemiluminescence method for the quantitative detection of endotoxin.Methods BALB/c mice were immunized with bacterial endotoxin as immunogen to prepare the required monoclonal antibody. The best antibody pairs were screened, biotin and acridinesulfonamide were labeled, and the samples were quantitatively detected by automatic chemiluminescence detector. The blank limit LoB, detection limit LoD and quantification limit LoQ were evaluated and verified, and the influence of fungal (1,3) -β-D glucan on endotoxin detection was evaluated.Results The blank limit LoB was set at 0.005EU/mL, the detection limit LoD was set at 0.010EU/mL, and the limit of quantification LoQ was set at 0.020 EU/mL. Fungal (1,3) -β-D glucan had no effect on endotoxin detection.Conclusion Chemiluminescence method can be used for the quantitative detection of bacterial endotoxin.
提供机构：
丹娜生物科技股份有限公司; Tianjin University of Science and Technology; Yaonan Lin
创建时间：
2024-03-20
