Transcriptome-wide analysis of wounded region gene expression using detached first-pair rosette leaves before culture (time 0) and 2 day after culture (DAC) from 12-day-old Col-0 seedlings

To reveal the molecular mechanism during de novo root regeneration from Arabidopsis leaf explants cultured on B5 medium without exogenous hormones, we carried out an RNA-seq experiment using detached leaf explants with partial petiole before culture (i.e. time 0) and 2 d after culturing (DAC) from12-d-old Col-0 seedlings. Gene expression of the wounded region (including the partial petiole and some surrounding tissues), which comprises regeneration-competent cells, was analyzed. Arabidopsis thaliana Col-0 seeds were grown on 1/2 MS medium with sucrose at 22 °C under a 16-h light/8-h dark photoperiod. Detached leaf explants with partial petiole from 12-day-old seedlings were cultured on B5 medium with sucrose at 22 °C under dark conditions for 0 (time 0) or 2 days (2 DAC). The wounded region (including the partial petiole and some surrounding tissues), which comprises regeneration-competent cells, was separated and collected for RNA extraction.