Probing cell identity hierarchies by fate titration and collision during direct reprogramming [scRNA-Seq]

Collide-seq: A systematic comparison of how different reprogramming transcription factors achieve conversion as well as an in-depth analysis of how cells resolve the conflict when more than one fate is instructed. Mouse embryonic fibroblasts were nucleofected with doxycycline inducible constructs for the indicated reprogramming transcription factors. Cells were expanded for about 10 days and positive cells sorted using FACS. All experimental conditions were pooled and transcription factor expression induced for the indicated time points before scRNA-seq.