HCN/CN− concentrations in PAO1 WT supernatantsa.

µg/ml LL-37 or no LL-37 (control) for 2 h at 37°C. Cell densities after 2 h peptide treatment were comparable in treated samples and controls, indicating no growth inhibition by LL-37. Supernatants were prepared by centrifugation following polarographic determination of HCN/CN− content.a Mid-log phase cultures of PAO1 WT were grown in MH broth containing either 20 = 9). Statistical significance of differences between mean values was confirmed by a two-sided t-test for independent samples (pb Mean averages and pooled standard deviations of three experiments, each measured in triplicate (n