Long-range effects of centromeres on the binding of the meiotic chromosome axis factor Red1

A chromosome size-dependent bias in meiotic recombination is in place to ensure that homologous chromosome pairing occurs for all chromosomes, including the smallest ones. This bias is clearly detectable during the assembly of the meiotic protein axis, the structure that compacts meiotic chromosomes and promotes recombination.To investigate the origin of the size bias, we mapped genome-wide occupancy of the meiotic axis protein Red1 in yeast strains containing chromosome fusions and synthetic chromosomes. Meiosis studies of the fusion and synthetic chromosomes further revealed that core centromeres influence the deposition of the axial element protein Red1 over distances >100kb, while pericentromeric regions co-evolved to reduce Red1 binding near centromeres and spread out Red1 along the chromosomes. Overall design: ChIP-seq analysis of Red1 DNA binding 3 hrs after induction of synchronous meiosis in SK1/S288C hybrid budding yeast strains containing fusions of SK1 chromosomes. Grant: March of Dimes FY16-208 Title: Meiotic segregation of small chromosomes PI: Andreas Hochwagen, New York University, New York, NY, United States Grant: NSF MCB-1616111 Title: Complete synthesis of designer eukaryotic genome, Sc2.0 PI: Jef D. Boeke, New York University Medical Center, New York, NY, United States