Induction of Immortal-like CAR-T Cells by Defined Factors (scRNA_seq)

In order to investigate the population heterogeneity of CAR19TIF, we determined to profile the single cell RNA transcriptional status of CAR19TIF cells derived from spleens of recipient mice at 2-month post cell transfer. Furthermore, we determined to examine potential subsets among the CAR19TIF cells and critical gene expression levels. Overall design: Thy1.1+ CAR19TIF cells derived from spleen of one recipient mouse were isolated and purified on 2-month post cell transfer. After sample preparation, scRNA seq was performed.