Single-cell analysis of circulating autoreactive CD4 T cells of autoimmune hepatitis patients (Figure 3)

The dominant intra-hepatic CD4 T cell clones found in the blood had an autoimmune and immuno-exhausted transcriptional profile transcriptomic signature We performed droplet-based single-cell RNA-seq combined with TCR sequencing of blood PD-1+ CXCR5- mCD4 T cells from four patients to characterize the transcriptional profile of cells with TCR clonotypes detected in the matched liver biopsies. PD-1+CD45RA-CXCR5- CD4 T cells from four distinct AIH patients were sorted on BD FACSAriaII. Cells were run on a Chromium Single Cell Controller using the Chromium Single Cell 5′ V2 Next GEM single cell kit