The effects of expression of mutant ASXL1 and HHEX on hematopoietic cells. The effects of expression of mutant ASXL1 and HHEX on hematopoietic cells

To identify target genes of mutant ASXL1 (ASXL1-MT) and HHEX in hematopoietic cells, we performed RNA-seq using RUNX1-ETO expressing cord blood cells transduced with vector or ASXL1-MT together with vector or HHEX. Overall design: Method: RUNX1-ETO expressing cord blood cells were transduced with control vector, ASXL1-MT (coexpressing GFP), HHEX (coexpressing NGFR) or ASXL1-MT+HHEX. 48hr after trunsduction, GFP+/NGFR+ cells were sorted by FACS. Total RNA extracted from sorted GFP+/NGFR+ cells were then subjected to RNA-seq analysis. Three biologically independent experiments were perfomed for each sample.