Skull vault formation in individual WT AB Fish

An individual fish was imaged successively from 12-42 dpf by confocal microscopy, during the period of induction and growth of the frontal and parietal bones. The fish was transgenic for col1a1a:gfp, marking chondrocytes, and for sp7:mcherry, marking osteoblasts.在额骨与顶骨的诱导及生长阶段，研究人员通过共聚焦显微镜（confocal microscopy）于受精后第12至42天（days post fertilization，dpf）对单条鱼进行连续成像。该鱼携带col1a1a:gfp与sp7:mcherry双转基因标记，其中col1a1a:gfp可标记软骨细胞（chondrocytes），sp7:mcherry可标记成骨细胞（osteoblasts）。