Understanding_the_Molecular_Basis_of_Egg_Hatching_in_Trichuris_Species. Understanding_the_Molecular_Basis_of_Egg_Hatching_in_Trichuris_Species

My goal is to understand the molecular mechanisms behind the hatching process in T. muris. Our preliminary data has shown us that it is only possible to induce hatching at specific time points during the 8 week long embryonation process, namely weeks 7 and 8. We want to study the transcriptome and proteome of T. muris eggs arrested during embryonation. Using bulk RNA-seq to compare the expression profiles of the eggs at 6 weeks where hatching is not observed and in mature eggs; observing potential important changes in expression that explain the ability of the larvae to respond to bacteria. Secondly, our data has shown that the rate of hatching varies in the presence of different E. coli strains. Our in house strain of E coli displays two phase dependant phenotypes a “rough” type and “smooth” type, and the rough type is poor at inducing hatching. We aim to understand the genetic basis for this variation by generating a high quality reference genome of the two types. This reference genome will also allow us to to screen a high volume of E. coli mutants using the TraDIS approach; once we have generated transposon mutant libraries we will use T. muris eggs to perform a “pull down” style assay and sequence the pools of mutants that are either successful or unsuccessful at interacting with the egg. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/