X-ray crystal structure of ALiS5-Streptavidine complex without cryo-protectant using a high-pressure cryocooling method
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X-ray crystal structure of ALiS5-Streptavidine complex without cryo-protectant using a high-pressure cryocooling method Descriptor: DIMETHYL SULFOXIDE, Streptavidin, dimethyl 5-(4-oxidanylidene-5~{H}-furo[3,2-c]pyridin-2-yl)benzene-1,3-dicarboxylate Authors: Higashiura, A, Yamamoto, A, Sugiyama, S. Deposit date: 2024-01-25 Release date: 2025-01-29 Last modified: 2025-12-24 Method: X-RAY DIFFRACTION (1.23 Å) Cite: Binding competition between substrates and cryoprotectants in protein crystals directly observed by a hybrid method of in-gel crystallization and high-pressure cryo-cooling in X-ray crystallography To Be Published
采用高压低温冷却法(high-pressure cryocooling method)制备的不含冷冻保护剂(cryo-protectant)的ALiS5-链霉亲和素(Streptavidine)复合物的X射线晶体结构 描述符:二甲基亚砜(DIMETHYL SULFOXIDE)、链霉亲和素(Streptavidin)、5-(4-氧代-5H-呋喃并[3,2-c]吡啶-2-基)苯-1,3-二羧酸二甲酯 作者:Higashiura A、Yamamoto A、Sugiyama S 提交日期:2024-01-25 发布日期:2025-01-29 最后修改日期:2025-12-24 实验方法:X射线衍射(分辨率1.23埃(Å)) 引用文献:《通过X射线晶体学中凝胶内结晶与高压低温冷却的混合方法直接观测蛋白质晶体中底物与冷冻保护剂的结合竞争》(待发表)



