Preparation of lutein through biotransformation in a microemulsion medium

To address the issues of low purity, poor stability, and environmental pollution associated with traditional organic solvent extraction methods for lutein production, and to meet the requirements for food-grade lutein in industrial applications, this study developed a microemulsion system using n-propyl acetate as the oil phase, Tween 80 as the surfactant, and ethanol as the co-surfactant. A microemulsion with 60% water content was successfully formulated as the reaction medium. Lipase was introduced to catalyze the hydrolysis of lutein esters, followed by optimization of key reaction parameters and evaluation of the stability of the resulting free lutein. The results demonstrated that under optimized conditions, the conversion rate of lutein ester reached 72.53 ± 2.80 %, representing a 153.87 % improvement compared to the 28.57 ± 1.81 % conversion achieved when using an acetonitrile-aqueous solution as the enzymatic reaction medium. Furthermore, the lutein microemulsion exhibited super..., , # Data from: Preparation of lutein through biotransformation in a microemulsion medium

Dataset DOI: [10.5061/dryad.ghx3ffc24](10.5061/dryad.ghx3ffc24)

This document presents the data and charts related to the research paper \"Preparation of lutein through biotransformation in a microemulsion medium.\"

## Description of the data and file structure

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“Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Figures.zip” contains 25 image files related to the data. The image format is tif.

“Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Data.zip\" contains 8 .csv files, which record all the measurement data in this article.

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### Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Figures.zip

Fig. 1, 2, 3, 9, and 11 in the compressed file were drawn using the Origin software. Fig. 4, 5, 6, 7, and 8 were obtained through the response surface optimizat..., ,