Preparation of lutein through biotransformation in a microemulsion medium

To address the issues of low purity, poor stability, and environmental pollution associated with traditional organic solvent extraction methods for lutein production, and to meet the requirements for food-grade lutein in industrial applications, this study developed a microemulsion system using n-propyl acetate as the oil phase, Tween 80 as the surfactant, and ethanol as the co-surfactant. A microemulsion with 60% water content was successfully formulated as the reaction medium. Lipase was introduced to catalyze the hydrolysis of lutein esters, followed by optimization of key reaction parameters and evaluation of the stability of the resulting free lutein. The results demonstrated that under optimized conditions, the conversion rate of lutein ester reached 72.53 ± 2.80 %, representing a 153.87 % improvement compared to the 28.57 ± 1.81 % conversion achieved when using an acetonitrile-aqueous solution as the enzymatic reaction medium. Furthermore, the lutein microemulsion exhibited super..., , # Data from: Preparation of lutein through biotransformation in a microemulsion medium Dataset DOI: [10.5061/dryad.ghx3ffc24](10.5061/dryad.ghx3ffc24) This document presents the data and charts related to the research paper \"Preparation of lutein through biotransformation in a microemulsion medium.\" ## Description of the data and file structure This repository contains two .zip compressed files. “Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Figures.zip” contains 25 image files related to the data. The image format is tif. “Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Data.zip\" contains 8 .csv files, which record all the measurement data in this article. And this Readme file. ### Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Figures.zip Fig. 1, 2, 3, 9, and 11 in the compressed file were drawn using the Origin software. Fig. 4, 5, 6, 7, and 8 were obtained through the response surface optimizat..., ,针对传统有机溶剂萃取法制备叶黄素时存在的纯度低、稳定性差、环境污染等问题，并满足工业应用对食品级叶黄素的需求，本研究构建了以乙酸正丙酯为油相、吐温80（Tween 80）为表面活性剂、乙醇为助表面活性剂的微乳液体系，成功制备出含水量60%的微乳液作为反应介质。本研究引入脂肪酶催化叶黄素酯水解，随后对关键反应参数进行优化，并对所得游离叶黄素的稳定性开展评价。结果表明，在优化条件下，叶黄素酯的转化率可达72.53 ± 2.80%，相较于以乙腈-水溶液作为酶促反应介质时的28.57 ± 1.81%转化率提升了153.87%。此外，本叶黄素微乳液展现出优异性能……（原文未完整）。 # 数据来源：《微乳液介质中生物转化法制备叶黄素》 数据集DOI：[10.5061/dryad.ghx3ffc24](10.5061/dryad.ghx3ffc24) 本数据集包含与研究论文《微乳液介质中生物转化法制备叶黄素》相关的实验数据与图表。 ## 数据与文件结构说明 本仓库包含两个压缩zip文件。 "Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Figures.zip"包含25份与本研究相关的图像文件，图像格式为tif。 "Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Data.zip"包含8个.csv文件，记录了本文中的全部实验测量数据。 此外还包含本自述文件。 ### "Preparation_of_lutein_through_biotransformation_in_a_microemulsion_medium-Figures.zip" 压缩包内的图1、图2、图3、图9和图11均使用Origin软件绘制。压缩包内的图4、图5、图6、图7和图8通过响应面优化……（原文未完整）。