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Expression data from fibroblasts with knock-down of Brg1 and Brm

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE142076
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We used microarrays to asses gene expression in 3T3 fibroblasts, after knock-down of Brg1 and Brm using stable shRNA interference. Cells were treated with 5ng/ml mouse TNF-alpha to stimulate inducible gene activation. 3T3 fibroblasts were transduced with retroviral vectors encoding an shRNA targeting Brg1 and Brm. Subconfluent cultures of cells were treated for 1hr with TNF-alpha before RNA extraction.
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2020-03-09
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