RNA-seq analysis of genes differentially expressed in NCI-H1299 cells with platensimycin (PTM) derivative 6p treatment

In order to characterize the global transcriptional effect and evaluate the impact of PTM derivative 6p on non-small cell lung cancer NCI-H1299 cell gene expression. NCI-H1299 cells were treated with vehicle (0.1% DMSO) or 25 µM 6p for 48 h and assessed the gene expression pattern by RNA-Seq. Finally, we show that extensive, coordinated effects of 6p on the transcriptional activity of genes within pathways controlling tumor cell metabolism, proliferation, and survival. Especially, the expression of key genes in de novo lipid synthesis were significantly reduced. Overall design: NCI-H1299 cells were cultured in 10% serum medium. Six samples obtained from NCI-H1299 cells including vehicle (0.1% DMSO) and 25 µM 6p treatment group were analyzed. Three independent samples were analyzed for each group.