Targeting Glutamine Synthetase in Tumors Disrupts Tumor Microenvironment-Regulated Cancer Cell Growth. Homo sapiens

Analysis of ovarian cancer cell line HeyA8 coculturing with cancer associated fibroblasts at gene expression level. The hypothesis test in this study is to show that cancer associated fibroblasts can influence the gene expression of HeyA8 under glutamine deprivation condition. Results provide important information of the response of HeyA8 when coculturing with cancer associated fibroblasts under glutamine deprivation, such as cell proliferation, apoptosis Overall design: Total RNA obtained from Hey8A cocultured with cancer associated fibroblasts subjected to 24 hours glutamine deprivation compared to He8A subjected 24 hours glutamine deprivation and Hey8A with complete medium condition