NanoSIMS depth profiles, O- & Cs+ beam

Data for Figures 3 and S6 in paper: "Elucidating Heterogeneous Iron Biomineralization Patterns in a Denitrifying As(III)-Oxidizing Bacterium: Implications for Arsenic Immobilization"<br>Single Acidovorax sp. ST3 cells (7 days incubation) were selected for depth profiling. Both Cs⁺ and O^- were used as primary ions and the selected areas were not implanted prior to starting the analysis (to preserve and keep the biomineral coating on the cells intact). Image raster sizes were 3-7 µm width. Spatial resolution was achieved by using D1=4 or 5 (beam size=120-100 nm). Pixel sizes were 128 x 128 or 256 x 256, and dwell time varied from 5,000-20,000 µs px^-1. 50-170 planes were collected, and the scanning was stopped when the ¹²C¹⁴N or ⁵⁶Fe⁺signal disappeared. The data was processed using the L’image software (Larry Nittler, Carnegie Institution of Washington) and the plugin OpenMIMS for ImageJ (www.nrims.harvard.edu). 3D reconstructions of the depth profiles were generated using the Thermo Scientific™ Avizo™ Software 9.7.0. Stack data of the negative secondary ions¹²C^-, ⁵⁶Fe¹⁶O^- and ⁷⁵As^-, and the positive secondary ions ²³Na⁺, ⁵⁶Fe⁺, and ⁷⁵As⁺, were first extracted in ImageJ and saved as “.raw” format files, which were loaded into Avizo™. The Z depth was compressed to 15-20 %. The ¹²C^- and ²³Na⁺signals were smoothed over 2-3 pixels. The commands “generate surface” and “show surface” were used sequentially, and the “transparent” display with a transparency of 80 % was selected. ⁵⁶Fe⁺ and ⁵⁶Fe¹⁶O^- signals were smoothed to 2 pixels and displayed as “shaded”. The ⁷⁵As^+/-ion counts were smoothed to 1 pixel.<br>Four depth profile files are presented, including the .im raw nanoSIMS files, extracted ⁷⁵As^+/-, ⁵⁶Fe¹⁶O^-/⁵⁶Fe⁺, ¹²C/²³Na counts as .raw format (ready to load into AVIZO), and complementary image and videos of the 3D reconstructions for each depth profile (jpg and mpg formats).